凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Prothrombin Fragment 1+2 (F1+2) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA710Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.2-200ng/mL
- 靈敏度最小可檢測劑量小于等于0.067 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 80-93 | 86 |
| heparin plasma(n=5) | 80-91 | 80 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-96% | 86-101% | 89-104% | 80-93% |
| EDTA plasma(n=5) | 91-98% | 78-99% | 80-99% | 93-101% |
| heparin plasma(n=5) | 91-98% | 81-104% | 97-105% | 83-104% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA710Ra01 | 凝血酶原片段F1+2(F1+2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA710Ra01 | 凝血酶原片段F1+2(F1+2)多克隆抗體 | WB |
| ULAA710Ra81 | 凝血酶原片段F1+2(F1+2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA710Ra71 | 凝血酶原片段F1+2(F1+2)多克隆抗體(生物素標記) | WB |
| USEA710Ra | 凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA710Ra | 凝血酶原片段F1+2(F1+2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA710Ra | 凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA710Ra01 | 凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine | High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study [Sjtrem:1757-7241] |
| Journal of Thrombosis and Haemostasis | High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality [Wiley: source] |
| British Journal of Dermatology | Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria [PubMed: 21910697] |
| Arteriosclerosis, Thrombosis, and Vascular Biology. | Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status [PubMed: 22859493] |
| Journal of Vascular Surgery | Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [PubMed: 23140799] |
| PLoS ONE | Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension [Plosone: Source] |
| Journal of Vascular Surgery | Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [Pubmed: 23140799 ] |
| Haemetology | Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124] |
| Cogent Medicine | Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction [Tandfonline:Source] |
| Interact Cardiovasc Thorac Surg. | An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose [Pubmed:24632424] |
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| J Thromb Haemost | Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism [PubMed: 26514101] |
| Acta Oncologica Turcica | Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer [aot:AOT_49_1_6_12.pdf] |
| Advances in Medical Sciences | Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation [pubmed:29145170] |
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| Acta?Haematologica | Elevations of Thrombotic Biomarkers in Hemoglobin H Disease [Pubmed:29402840] |
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