血管緊張素原(AGT)單克隆抗體
Monoclonal Antibody to Angiotensinogen (AGT)
ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
- 編號UMAA797Ra24
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C5
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA797Ra01-血管緊張素原(AGT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 326 ¥ 760 ¥ 1086 ¥ 2715 ¥ 10860
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Liver Tissue Primary Ab: 30μg/ml Mouse Anti-Rat AGT Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Liver lysate; Lane3: Rat Kidney lysate Primary Ab: 3μg/ml Mouse Anti-Rat AGT Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對AGT的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別AGT。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA797Ra61 | 血管緊張素原(AGT)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA797Ra01 | 血管緊張素原(AGT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA797Ra61 | 血管緊張素原(AGT)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA797Ra01 | 血管緊張素原(AGT)多克隆抗體 | WB |
| ULAA797Ra71 | 血管緊張素原(AGT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA797Ra81 | 血管緊張素原(AGT)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA797Ra25 | 血管緊張素原(AGT)單克隆抗體 | ICC/IF |
| UMAA797Ra21 | 血管緊張素原(AGT)單克隆抗體 | WB; IHC |
| UMAA797Ra22 | 血管緊張素原(AGT)單克隆抗體 | WB |
| UMAA797Ra23 | 血管緊張素原(AGT)單克隆抗體 | WB |
| UMAA797Ra24 | 血管緊張素原(AGT)單克隆抗體 | WB; IHC |
| UMAA797Ra27 | 血管緊張素原(AGT)單克隆抗體 | IHC |
| UMAA797Ra26 | 血管緊張素原(AGT)單克隆抗體 | WB; ICC/IF |
| UFAA797Ra02 | 抗血管緊張素原(AGT)單克隆抗體 | FCM |
| USEA797Ra | 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA797Ra | 血管緊張素原(AGT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA797Ra01 | 血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Transplant International | Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? [Wiley: source] |
| Amyloid | The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients [PubMed: 22320202] |
| Pediatric Mephrology | Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension [PubMed: PMC3661928] |
| Acta Paediatrica | Urinary angiotensinogen as a novel marker of obstructive nephropathy in children [Pubmed: 23772991] |
| Journal of the Renin-Angiotensin-Aldosterone System | Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia [Sagepub: Source] |
| Pediatric Nephrology | MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY [Oxfordjournals:Source] |
| journal of human hypertension | Relationship between blood pressure var [PubMed: 26223347] |
| PLoS One | Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis [PubMed: 26098644] |
| MKA | Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa [Index.Php: Art] |
| Journal of Human Hypertension | Relationship between blood pressure variability and renal activity of the renin-angiotensin system. [pubmed:26223347] |
| Acta Pharmacol Sin. | Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Life?Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488] |
| American Journal of Physiology-Renal?Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109] |
| Methods in Molecular Biology | Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia [Pubmed: 31069778] |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
| Hypertens Res | Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792] |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy?\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/|??\catenin signaling [34057312] |
| Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253] |
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